+ 44 (0) 1709 361 241 info@kirkstall.com
Published Papers using Quasi Vivo

Publications featuring Quasi Vivo®.

  1. Evaluating the impact of dynamic fluid flow on an advanced model of pancreatic cancer: Towards desmoplasia disruption and chemosensitivity promotion
    Priyanka Gupta, John Richardson, Malcolm Wilkinson, Oleksiy V. Klymenko, Deniz Bakkalci, Umber Cheema, Hemant M. Kocher, Pedro A. Pérez-Mancera, Eirini G. Velliou
  2. Kirkstall Ltd, Supporting Emerging Standards for Organ on a Chip
    https://kirkstall.com/supporting-emerging-standards-for-organ-on-a-chip/
  3. Kirkstall Ltd, A Brief Introduction to Flow (2025) [White Paper]
    https://kirkstall.com/an-introduction-to-perfusion-flow-cell-culture/
  4. Exploring novel in vitro tools to unravel gut-blood-brain communication
    Laure Maes
  5. Ex Vivo Placental Explant Flow Culture – Mimicking the Dynamic Conditions In Utero
    Nadja Kupper, Elisabeth Pritz, Monika Siwetz, Jacqueline Guettler, Berthold Huppertz
  6. Modeling of lung-liver interaction during infection in a human microfluidic organ-on-a-chip
    Susanne Reinhold, View ORCID ProfileChristian Herr, Yiwen Yao, Mehdi Pourrostami, Felix Ritzmann, Thorsten Lehr, Dominik Selzer, Yvonne Kohl, Daniela Yildiz, Hortense Slevogt, View ORCID ProfileChristoph Beisswenger, Robert Bals
    doi: https://doi.org/10.1101/2023.06.01.543192
  7. Wilkinson, JM. A review of complex in vitro cell culture stressing the importance of fluid flow and illustrated by organ on a chip liver models. Front. Toxicol 5 (2023). https://doi.org/10.3389/ftox.2023.1170193
  8. Mäki-Mikola, E., Lauren, P., Uema, N. et al.Establishing a simple perfusion cell culture system for light-activated liposomes. Sci Rep 13, 2050 (2023). https://doi.org/10.1038/s41598-023-29215-6
  9. Meldrum, K., Amaral Duarte De Moura, J., Doak, S., & Clift, M. (2022). Dynamic Fluid Flow Exacerbates the (Pro-)Inflammatory Effects of Aerosolised Engineered Nanomaterials In Vitro. Nanomaterials, 12(19), 3431. https://doi.org/10.3390/nano12193431
  10. Spencer CE, Rumbelow S, Mellor S, Duckett CJ, Clench MR. Adaptation of the Kirkstall QV600 LLI Microfluidics System for the Study of Gastrointestinal Absorption by Mass Spectrometry Imaging and LC-MS/MS. Pharmaceutics. 2022; 14(2):364. https://doi.org/10.3390/pharmaceutics14020364
  11. Kupper N, Pritz E, Siwetz M, Guettler J, Huppertz B. Placental Villous Explant Culture 2.0: Flow Culture Allows Studies Closer to the In Vivo Situation. International Journal of Molecular Sciences. 2021; 22(14):7464. https://doi.org/10.3390/ijms22147464
  12. Geddes L, Themistou E, Burrows JF, Buchanan FJ, Carson L. Evaluation of the in vitro cytotoxicity and modulation of the inflammatory response by the bioresorbable polymers poly(D,L-lactide-co-glycolide) and poly(L-lactide-co-glycolide). Acta Biomater. 2021; 134:261-275. doi: 1016/j.actbio.2021.07.049
  13. Azimi T, Loizidou M, Dwek MV. Cancer cells grown in 3D under fluid flow exhibit an aggressive phenotype and reduced responsiveness to the anti-cancer treatment doxorubicin. Sci Rep. 2020;10(1):12020. https://doi.org/10.1038/s41598-020-68999-9
  14. Elbakary B, Badhan RKS. A dynamic perfusion based blood-brain barrier model for cytotoxicity testing and drug permeation. Sci Rep. 2020 Mar 2;10(1):3788. https://doi.org/10.1038/s41598-020-60689-w
  15. Hyndman L, McKee S, Mottram NJ, Singh B, Webb SD and McGinty S. Mathematical modelling of fluid flow and solute transport to define operating parameters for in vitroperfusion cell culture systems. Interface Focus, 2020, 10(2). https://doi.org/10.1098/rsfs.2019.0045
  16. Keable A, O’Neill R, MacGregor Sharp M, Gatherer M, Yuen HM, Johnston DA, Weller RO, Carare RO. ApoE4 Astrocytes Secrete Basement Membranes Rich in Fibronectin and Poor in Laminin Compared to ApoE3 Astrocytes. Int J Mol Sci. 2020; 21(12):4371. https://doi.org/10.3390/ijms21124371
  17. Tomlinson L, Hyndman L, Firman JW, Bentley R, Kyffin JA, Webb SD, McGinty S, Sharma P. In vitroLiver Zonation of Primary Rat Hepatocytes. Front BioengBiotechnol. 2019; 7(17). https://doi.org/10.3389/fbioe.2019.00017
  18. Ahmed, S., Chauhan, V.M., Ghaemmaghami, A.M. et al.New generation of bioreactors that advance extracellular matrix modelling and tissue engineering. Biotechnol Lett 41, 1–25 (2019). https://doi.org/10.1007/s10529-018-2611-7
  19. Miranda-Azpiazu P, Panagiotou S, Jose G, Saha S. A novel dynamic multicellular co-culture system for studying individual blood-brain barrier cell types in brain diseases and cytotoxicity testing. Sci Rep. 2018, 8(1). https://doi.org/10.1038/s41598-018-26480-8
  20. Berger E, Magliaro C, Paczia N, Monzel AS, Antony P, Linster CL, Bolognin S, Ahluwalia A, Schamborn JC. Millifluidic culture improves human midbrain organoid vitality and differentiation. Lab Chip, 2018, 18, 3172-3183. https://doi.org/10.1039/C8LC00206A
  21. Buesch, Stefanie & Schroeder, Jenny & Bunger, Maureen & D’Souza, Theresa &Stosik, Magdalene. (2018). A Novel In Vitro Liver Cell Culture Flow System Allowing Long-Term Metabolism and Hepatotoxicity Studies. Applied In Vitro Toxicology. 4. https://doi.org/10.1089/aivt.2018.0009
  22. Chandorkar P, Posch W, Zaderer V et al.Fast-track development of an in vitro 3D lung/immune cell model to study Aspergillus Sci Rep 7, 11644 (2017). https://doi.org/10.1038/s41598-017-11271-4
  23. Martin K, Yuan X, Stimac G, Bannerman K, Anderson J, Roy C, Glykofrydis F, Yin H and Davies J (2017). Symmetry-breaking in branching epithelia: cells on micro-patterns under flow challenge the hypothesis of positive feedback by a secreted autocrine inhibitor of motility. Journal of Anatomy, 230(6), 766-774. https://doi.org/10.1111/joa.12599
  24. Rashidi H, Alhaque S, Szkolnicka D, Flint O and Hay D (2016). Fluid shear stress modulation of hepatocyte-like cell function. Archives of Toxicology, 90(7), 1757-1761. https://doi.org/10.1007/s00204-016-1689-8
  25. Nithiananthan S, Crawford A, Knock J, Lambert D and Whawell S (2016). Physiological Fluid Flow Moderates Fibroblast Responses to TGF-β1. Journal of Cellular Biochemistry, 118(4), 878-890. https://doi.org/10.1002/jcb.25767
  26. Pedersen J, Shim Y, Hans V, Phillips M, Macdonald J, Walker G, Andersen M, Clewell H and Yoon M (2016). Fluid Dynamic Modeling to Support the Development of Flow-Based Hepatocyte Culture Systems for Metabolism Studies. Frontiers in Bioengineering and Biotechnology, 4, 72. https://doi.org/10.3389/fbioe.2016.00072
  27. Shannahan J, Fritz K, Raghavendra A, Podila R, Persaud I and Brown J (2016). Disease-Induced Disparities in Formation of the Nanoparticle-Biocorona and the Toxicological Consequences. Toxicological Sciences, 152(2), 406-416. https://doi.org/10.1093/toxsci/kfw097
  28. Faure M, Guibert E, Alves S, Pain B, Ramé C, Dupont J, Brillard J and Froment P (2016). The insulin sensitiser metformin regulates chicken Sertoli and germ cell populations. Reproduction, 151(5), 527-538. https://doi.org/10.1530/REP-15-0565
  29. Ramachandran S, Schirmer K, Münst B, Heinz S, Ghafoory S, Wölfl S, Simon-Keller K, Marx A, Øie C, Ebert M, Walles H, Braspenning J and Breitkopf-Heinlein K (2015). In Vitro Generation of Functional Liver Organoid-Like Structures Using Adult Human Cells. PLoS ONE, 10(10), e0139345. https://doi.org/10.1371/journal.pone.0139345
  30. Ucciferri N, Collnot EM, Gaiser BK, Tirella A, Stone V, Domenici C, Lehr CM, Ahluwalia A. In vitro toxicological screening of nanoparticles on primary human endothelial cells and the role of flow in modulating cell response. Nanotoxicology, 2014; 8(6):697-708. https://doi.org/10.3109/17435390.2013.831500
  31. Giusti, S., Sbrana, T., La Marca, M., Di Patria, V., Martinucci, V., Tirella, A., Domenici, C. and Ahluwalia, A. (2014). A novel dual-flow bioreactor simulates increased fluorescein permeability in epithelial tissue barriers. Biotechnology Journal, 9(9). 1002/biot.201400004
  32. Pagliari S, Tirella A, Ahluwalia A, Duim S, Goumans M, Aoyagi T and Forte G (2014). A multistep procedure to prepare pre-vascularized cardiac tissue constructs using adult stem cells, dynamic cell cultures, and porous scaffolds. Frontiers in Physiology, 5(June), 210. https://doi.org/10.3389/fphys.2014.00210
  33. Mattei G, Giusti S and Ahluwalia A (2014). Design Criteria for Generating Physiologically Relevant In Vitro Models in Bioreactors. Processes, 2(3), 548-569. https://doi.org/10.3390/pr2030548
  34. Iori, E., Vinci, B., Murphy, E., Marescotti, M., Avogaro, A. and Ahluwalia, A. (2012). Glucose and Fatty Acid Metabolism in a 3 Tissue In-Vitro Model Challenged with Normo- and Hyperglycaemia. PLoS ONE, 7(4). https://doi.org/10.1371/journal.pone.0034704
  35. Vinci B, Duret C, Klieber S, Gerbal-Chaloin S, Sa-Cunha A, Laporte S, Suc B, Maurel P, Ahluwalia A and Daujat-Chavanieu M (2011). Modular bioreactor for primary human hepatocyte culture: Medium flow stimulates expression and activity of detoxification genes. Biotechnology Journal, 6(5), 554-564. https://doi.org/10.1002/biot.201000326
  36. Vozzi F, Mazzei D, Vinci B, Vozzi G, Sbrana T, Ricotti L, Forgione N and Ahluwalia A (2011). A flexible bioreactor system for constructing in vitro tissue and organ models. Biotechnology and Bioengineering, 108(9), 2129-2140. ttps://doi.org/10.1002/bit.23164
  37. Vinci B, Murphy E, Iori E, Meduri F, Fattori S, Marescotti M, Castagna M, Avogaro A and Ahluwalia A (2011). An in vitro model of glucose and lipid metabolism in a multicompartmental bioreactor. Biotechnology Journal, 7(1), 117-126. https://doi.org/10.1002/biot.201100177
  38. Mazzei D, Guzzardi M, Giusti S, and Ahluwalia A (2010). A low shear stress modular bioreactor for connected cell culture under high flow rates. Biotechnology and Bioengineering, 106(1), 127–137. https://doi.org/10.1002/bit.22671

Quasi Vivo®, used in 100+ labs worldwide, getting started is easy and cost effective!

ENQUIRE NOW

Sign Up For Our Newsletter

For all the latest from us, including interesting research videos and more, please subscribe to our newsletter below.
All data is stored in accordance with our privacy policy .

    Phone: + 44 (0) 1709 361 241

    Business Hours: Monday to Friday 9:00am-5:00pm (UK Time)